Pronuclear embryo transfer consists of performing in vitro fertilization using the eggs of the affected woman whose mitochondria contain mutant DNA and the sperm of the future father, and subsequent extraction of the pronucleus on day 1 of development, leaving behind most of the mutated mitochondria. These pronuclei are transferred to an enucleated zygote with healthy mitochondria. They are transferred to an enucleated zygote, not an egg since the developmental state must be the same. The hybrid zygote is then developed in vitro until it reaches an appropriate state for transfer to the uterus.

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